1AUW
H91N DELTA 2 CRYSTALLIN FROM DUCK
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.100, 100.200, 109.100 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TURKEY DELTA 1 CRYSTALLINE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 19.920 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.103 * | 0.330 * |
| Total number of observations | 239855 * | |
| Number of reflections | 65641 | |
| Completeness [%] | 95.0 | 92.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 18-20% PEG MME 2K, 300 MM MGCL2,100 MM TRIS-HCL PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | PEG MME2000 | 9-10 (%(w/v)) | |
| 4 | 1 | drop | 150 (mM) | ||
| 5 | 1 | drop | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | PEG MME2000 | 18-20 (%(w/v)) | |
| 7 | 1 | reservoir | 300 (mM) | ||
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) |
