1AUW

H91N DELTA 2 CRYSTALLIN FROM DUCK

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	1995-01
Detector	FUJI
Spacegroup name	P 1 21 1
Unit cell lengths	94.100, 100.200, 109.100
Unit cell angles	90.00, 102.10, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.161
Rwork	0.161
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TURKEY DELTA 1 CRYSTALLINE
RMSD bond length	0.006
RMSD bond angle	19.920 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.103 *	0.330 *
Total number of observations	239855 *
Number of reflections	65641
Completeness [%]	95.0	92.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		PROTEIN WAS CRYSTALLIZED FROM 18-20% PEG MME 2K, 300 MM MGCL2,100 MM TRIS-HCL PH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-8 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	PEG MME2000	9-10 (%(w/v))
4	1	drop		150 (mM)
5	1	drop	Tris-HCl	50 (mM)
6	1	reservoir	PEG MME2000	18-20 (%(w/v))
7	1	reservoir		300 (mM)
8	1	reservoir	Tris-HCl	100 (mM)