1AUE

FKBP-RAPAMYCIN BINDING DOMAIN (FRB) OF THE FKBP-RAPAMYCIN ASSOCIATED PROTEIN

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1997-02
Detector	PRINCETON 2K
Spacegroup name	P 21 21 21
Unit cell lengths	29.940, 59.210, 123.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.330
R-factor	0.232
Rwork	0.232
R-free	0.33900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fap
RMSD bond length	0.018
RMSD bond angle	1.858 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.420
High resolution limit [Å]	2.330	2.330
Rmerge	0.032 *
Number of reflections	7307
<I/σ(I)>	6567.9	1324
Completeness [%]	73.0	28.9
Redundancy	1.73	1.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	HANGING DROPS AT 4 C, pH 8.0, vapor diffusion - hanging drop, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-EDTA	50 (mM)
3	1	drop	2-MPD	20 (%(w/v))
4	1	drop	PEG4000	10 (%(w/v))