1AU1

HUMAN INTERFERON-BETA CRYSTAL STRUCTURE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	108
Detector technology	IMAGE PLATE
Collection date	1996-01-10
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	55.300, 65.900, 121.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.223
Rwork	0.223
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.580
Data reduction software	bioteX
Phasing software	AMoRE
Refinement software	X-PLOR (3.81)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Rmerge	0.077
Number of reflections	18405
Completeness [%]	83.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	sodium acetate	25 (mM)
3	1	reservoir	PEG4000	14 (%)
4	1	reservoir	sodium acetate	0.1 (M)
5	1	reservoir	ammonium acetate	0.2 (M)