1AT5

HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-05
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	78.610, 78.610, 38.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.182
Rwork	0.182
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lzb
RMSD bond length	0.010
RMSD bond angle	23.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.800	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.052
Total number of observations	56262 *
Number of reflections	10924
Completeness [%]	92.3	65.2
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.2	4 *	CRYSTALS WERE PREPARED AT 277K FROM A SOLUTION CONTAINING 20 MG/ML PROTEIN, 7 MM TRI-N-ACETYLCHITOTRIOSE, 25 MG/ML SODIUM CHLORIDE, AND 100 MM ACETATE BUFFER PH 4.2, BY EQUILIBRATING THE SOLUTION AGAINST A RESERVOIR SOLUTION CONTAINING 50 MG/ML SODIUM CHLORIDE AND 100 MM ACETATE BUFFER PH 4.2.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	NAG3	7 (mM)
3	1	drop		430 (mM)
4	1	drop	sodium acetate	100 (mM)
5	1	reservoir		860 (mM)
6	1	reservoir	sodium acetate	100 (mM)