1AT5
HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | RIGAKU |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.610, 78.610, 38.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lzb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.800 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | |
| Total number of observations | 56262 * | |
| Number of reflections | 10924 | |
| Completeness [%] | 92.3 | 65.2 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.2 | 4 * | CRYSTALS WERE PREPARED AT 277K FROM A SOLUTION CONTAINING 20 MG/ML PROTEIN, 7 MM TRI-N-ACETYLCHITOTRIOSE, 25 MG/ML SODIUM CHLORIDE, AND 100 MM ACETATE BUFFER PH 4.2, BY EQUILIBRATING THE SOLUTION AGAINST A RESERVOIR SOLUTION CONTAINING 50 MG/ML SODIUM CHLORIDE AND 100 MM ACETATE BUFFER PH 4.2. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | NAG3 | 7 (mM) | |
| 3 | 1 | drop | 430 (mM) | ||
| 4 | 1 | drop | sodium acetate | 100 (mM) | |
| 5 | 1 | reservoir | 860 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 100 (mM) |
