1AQP

RIBONUCLEASE A COPPER COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1996-10
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	58.180, 53.800, 42.900
Unit cell angles	90.00, 118.90, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.2
Rwork	0.200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rsa
RMSD bond length	0.010
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.038	0.080
Number of reflections	6930
<I/σ(I)>	13	9
Completeness [%]	85.0	55
Redundancy	2.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5		ENZYME WAS CRYSTALLIZED FROM MPD WITH 6 MOLAR EXCESS OF COPPER, pH 5.5