1AOW
ANNEXIN IV
Experimental procedure
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1997-02 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 118.560, 118.560, 82.233 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 * - 2.990* |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ann |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.570 |
| Data reduction software | XDS |
| Data scaling software | MARSCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 3.120 |
| High resolution limit [Å] | 2.990 * | 2.990 |
| Rmerge | 0.049 | 0.070 |
| Number of reflections | 8114 | |
| <I/σ(I)> | 36 | 10 |
| Completeness [%] | 93.0 * | 87 * |
| Redundancy | 5.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | SITTING DROP, FROM A PROTEIN SOLUTION 10 MG/ML, IN THE PRESENCE OF 1.4 M AMMONIUM SULFATE, 10 MM BETA-MERCAPTOETHANOL, PH=5.0, vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1 (M) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 5 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) | |
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) |
