Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-02-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.800, 77.400, 87.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.21800 |
| Structure solution method | MAD/MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.099 * | 0.278 * |
| Number of reflections | 61005 | |
| <I/σ(I)> | 28.7 | 7.5 |
| Completeness [%] | 96.6 | 93.5 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.7 | pH 7.7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | potassium phosphate | 65 (mM) | |
| 3 | 1 | reservoir | EDTA | 0.1 (M) | |
| 4 | 1 | reservoir | PEG8000 | 15 (%) |
