1AOJ

THE SH3 DOMAIN OF EPS8 EXISTS AS A NOVEL INTERTWINED DIMER

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1996-02
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1
Unit cell lengths	27.690, 28.338, 36.825
Unit cell angles	107.53, 96.83, 104.51

Refinement procedure

Resolution	32.000 - 2.500
R-factor	0.193 *
Rwork	0.193
R-free	0.27700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1shf
RMSD bond length	0.005
RMSD bond angle	22.352 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4 ((ALMN))
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.106	0.397
Total number of observations	21281 *
Number of reflections	3406
<I/σ(I)>	12.6	3.3
Completeness [%]	97.0	92
Redundancy	6.2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		PROTEIN WAS CRYSTALLIZED FROM 0.4M SODIUM POTASSIUM TARTRATE, PH 7.5. PROTEIN CONC. 6MG/ML. CRYSTALLIZATION TIME 2-3 WEEKS.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	drop	sodium potassium tartrate	0.2 (M)