1AOJ
THE SH3 DOMAIN OF EPS8 EXISTS AS A NOVEL INTERTWINED DIMER
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 27.690, 28.338, 36.825 |
Unit cell angles | 107.53, 96.83, 104.51 |
Refinement procedure
Resolution | 32.000 - 2.500 |
R-factor | 0.193 * |
Rwork | 0.193 |
R-free | 0.27700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shf |
RMSD bond length | 0.005 |
RMSD bond angle | 22.352 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 ((ALMN)) |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.106 | 0.397 |
Total number of observations | 21281 * | |
Number of reflections | 3406 | |
<I/σ(I)> | 12.6 | 3.3 |
Completeness [%] | 97.0 | 92 |
Redundancy | 6.2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | PROTEIN WAS CRYSTALLIZED FROM 0.4M SODIUM POTASSIUM TARTRATE, PH 7.5. PROTEIN CONC. 6MG/ML. CRYSTALLIZATION TIME 2-3 WEEKS. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3 (mg/ml) | |
2 | 1 | drop | sodium potassium tartrate | 0.2 (M) |