1ANX
THE CRYSTAL STRUCTURE OF A NEW HIGH-CALCIUM FORM OF ANNEXIN V
Experimental procedure
Spacegroup name | P 1 |
Unit cell lengths | 91.200, 91.300, 36.200 |
Unit cell angles | 82.40, 82.40, 118.20 |
Refinement procedure
Resolution | 12.000 - 1.900 |
R-factor | 0.198 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.050 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 12.000 * |
High resolution limit [Å] | 1.900 * |
Rmerge | 0.040 * |
Total number of observations | 184680 * |
Number of reflections | 74259 * |
Completeness [%] | 94.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | calcium | 10-60 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.8-2 (M) | |
5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
6 | 1 | drop | phospholipid | or a mixture of 0.1 %(w/v) agarose or 0.1 %(w/v) sucrose |