1AKZ
HUMAN URACIL-DNA GLYCOSYLASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 275 |
| Collection date | 1995-02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.760, 55.270, 84.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.570 |
| R-factor | 0.187 * |
| Rwork | 0.189 |
| R-free | 0.22100 * |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.960 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.046 | 0.103 * |
| Total number of observations | 121341 * | |
| Number of reflections | 30415 * | |
| <I/σ(I)> | 14.9 | 11 |
| Completeness [%] | 94.5 | 93.3 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.9 | Mol, C.D., (1995) Cell. 80, 869. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 16-20 (%) | |
| 3 | 1 | reservoir | imidazole/malate | 100 (mM) | pH7.9 |
| 4 | 1 | reservoir | 1-4 (%sat) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1-4 (%sat) |
