1AKZ

HUMAN URACIL-DNA GLYCOSYLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	275
Collection date	1995-02
Spacegroup name	P 21 21 21
Unit cell lengths	47.760, 55.270, 84.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.570
R-factor	0.187 *
Rwork	0.189
R-free	0.22100 *
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	23.960 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.570	1.570
Rmerge	0.046	0.103 *
Total number of observations	121341 *
Number of reflections	30415 *
<I/σ(I)>	14.9	11
Completeness [%]	94.5	93.3 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.9		Mol, C.D., (1995) Cell. 80, 869. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	PEG4000	16-20 (%)
3	1	reservoir	imidazole/malate	100 (mM)	pH7.9
4	1	reservoir		1-4 (%sat)
5	1	reservoir	ammonium sulfate	1-4 (%sat)