1AJ0

CRYSTAL STRUCTURE OF A TERNARY COMPLEX OF E. COLI DIHYDROPTEROATE SYNTHASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	289
Detector technology	AREA DETECTOR
Collection date	1993-07
Detector	SIEMENS
Spacegroup name	C 1 21 1
Unit cell lengths	92.910, 60.670, 59.820
Unit cell angles	90.00, 114.67, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.206 *
Rwork	0.206
RMSD bond length	0.010
RMSD bond angle	0.032
Data reduction software	XENGEN
Data scaling software	XENGEN
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.054 *
Total number of observations	66358 *
Number of reflections	23202
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.4		PROTEIN WAS CRYSTALLIZED FROM 42% AMMONIUM SULFATE, 50MM TRIS-HCL, PH 8.4.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	42 (%sat)
2	1	1	Tris-HCl	50 (mM)