1AB8
RAT TYPE II ADENYLYL CYCLASE C2 DOMAIN/FORSKOLIN COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | FUJI |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.300, 81.300, 180.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.28400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | |
| Total number of observations | 51618 * | |
| Number of reflections | 17326 | |
| <I/σ(I)> | 15 | 3 |
| Completeness [%] | 91.0 | 40 |
| Redundancy | 3.5 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.9 | 21-24 * | 1.1 M AM SO4, 0.1 M PHOSPHATE, PH 5.9, 2 % DMSO, 1 MM FORSKOLIN, 10 MM DTT, 80 MM NACL, 50 MM TRIS HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 17.5 (mg/ml) | |
| 2 | 1 | 2 | forskolin | 1 (mM) | |
| 3 | 1 | 2 | DMSO | 2 (%) | |
| 4 | 1 | 2 | Tris-HCl | 50 (mM) | |
| 5 | 1 | 2 | 80 (mM) | ||
| 6 | 1 | 2 | DTT | 10 (mM) | |
| 7 | 1 | 2 | ammonium sulphate | 1.1-1.2 (M) | |
| 8 | 1 | 2 | sodium phophate | 0.1 (M) |
