1AB8

RAT TYPE II ADENYLYL CYCLASE C2 DOMAIN/FORSKOLIN COMPLEX

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1996-12
Detector	FUJI
Spacegroup name	P 43 21 2
Unit cell lengths	81.300, 81.300, 180.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.219
Rwork	0.219
R-free	0.28400
Structure solution method	MIRAS
RMSD bond length	0.011
RMSD bond angle	24.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.067
Total number of observations	51618 *
Number of reflections	17326
<I/σ(I)>	15	3
Completeness [%]	91.0	40
Redundancy	3.5	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.9	21-24 *	1.1 M AM SO4, 0.1 M PHOSPHATE, PH 5.9, 2 % DMSO, 1 MM FORSKOLIN, 10 MM DTT, 80 MM NACL, 50 MM TRIS HCL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	17.5 (mg/ml)
2	1	2	forskolin	1 (mM)
3	1	2	DMSO	2 (%)
4	1	2	Tris-HCl	50 (mM)
5	1	2		80 (mM)
6	1	2	DTT	10 (mM)
7	1	2	ammonium sulphate	1.1-1.2 (M)
8	1	2	sodium phophate	0.1 (M)