1AAY
ZIF268 ZINC FINGER-DNA COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.400, 56.200, 130.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.600 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zaa WITHOUT WATERS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.292 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 0.534 |
| Total number of observations | 149720 * | |
| Number of reflections | 27503 * | |
| <I/σ(I)> | 34.5 | 2 |
| Completeness [%] | 94.8 * | 94.9 * |
| Redundancy | 6.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | Pavletich, N.P., (1991) Science, 252, 809. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Zif complex | 1 (mM) | |
| 2 | 1 | drop | 450-750 (mM) | ||
| 3 | 1 | drop | bis-tris-propane-HCl | 125 (mM) | |
| 4 | 1 | reservoir | PEG400 | 0-10 (%) | |
| 5 | 1 | reservoir | 450-650 (mM) | ||
| 6 | 1 | reservoir | bis-tris-propane-HCl | 25 (mM) |
