1A8B

RAT ANNEXIN V COMPLEXED WITH GLYCEROPHOSPHOETHANOLAMINE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-07
Detector	RIGAKU RAXIS
Spacegroup name	H 3
Unit cell lengths	157.470, 157.470, 37.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.2
Rwork	0.200
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.018
RMSD bond angle	21.300 *
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	44.700
High resolution limit [Å]	1.900
Rmerge	0.125
Total number of observations	39223 *
Number of reflections	18875
Completeness [%]	67.4
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *		PROTEIN CRYSTALLIZED FROM AMMONIUM SULFATE, 20MM CACL2, 50MM HEPES, PH 8.2; SOAKED IN 20% PEG, 20MM CACL2, 50MM GPE, PH 7.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-30 (mg/ml)
2	1	reservoir	ammonium sulfate	40-60 (%)
3	1	reservoir		5-10 (mM)
4	1	reservoir	HEPES	50 (mM)
5	1	reservoir	dithiothreitol	1 (mM)
6	1	reservoir	sodium azide