1A7H

GAMMA S CRYSTALLIN C-TERMINAL DOMAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	62.250, 62.250, 170.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.560
R-factor	0.208
Rwork	0.208
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GAMMA B C-TERMINAL
RMSD bond length	0.009
RMSD bond angle	26.130 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.640	2.650
High resolution limit [Å]	2.560	2.560
Rmerge	0.035	0.058
Total number of observations	33311 *
Number of reflections	6716
<I/σ(I)>	14.7	11.5
Completeness [%]	98.6	97.9
Redundancy	5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *	PROTEIN CONCENTRATION 5MGS/ML. 0.1M NA-CITRATE, PH 5.0, 20-22%(W/V) PEG8K, 2% 1,4-DIOXANE, AT ROOM-TEMPERATURE.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Mes/NaOH	25 (mM)
3	1	reservoir	PEG8000	11 (%(w/v))
4	1	reservoir	Na-citrate