1A7G

THE CRYSTAL STRUCTURE OF THE E2 DNA-BINDING DOMAIN FROM HUMAN PAPILLOMAVIRUS AT 2.4 ANGSTROMS

Experimental procedure

Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1996-10
Detector	RIGAKU
Spacegroup name	P 61 2 2
Unit cell lengths	45.890, 45.890, 195.636
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.211
Rwork	0.211
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BPV-1 STRUCTURE
RMSD bond length	0.014
RMSD bond angle	23.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.85)
Refinement software	X-PLOR (3.85)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.600
High resolution limit [Å]	2.400	2.400
Rmerge	0.065	0.185
Total number of observations	60427 *
Number of reflections	5649
<I/σ(I)>	4	2.5
Completeness [%]	90.0	88.4
Redundancy	2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-25 (mg/ml)
2	1	reservoir	citrate	100 (mM)
3	1	reservoir	ammonium sulfate	45-65 (%sat)