1A6R
GAL6 (YEAST BLEOMYCIN HYDROLASE) MUTANT C73A
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 150.676, 150.676, 90.163 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.050 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gcb |
RMSD bond length | 0.011 |
RMSD bond angle | 21.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.077 | 0.164 |
Total number of observations | 367565 * | |
Number of reflections | 37272 | |
<I/σ(I)> | 58 | 31 |
Completeness [%] | 97.3 | 99.2 |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.5 * | PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE AND PEG 4K., pH 9.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | bleomycin | 0.5 (mM) | |
3 | 1 | drop | Tris-HCl | 25 (mM) | |
4 | 1 | reservoir | PEG4000 | 10 (%) | |
5 | 1 | reservoir | ammonium sulfate | 0.45 (M) | |
6 | 1 | reservoir | Tris | 0.1 (M) |