1A6Q
CRYSTAL STRUCTURE OF THE PROTEIN SERINE/THREONINE PHOSPHATASE 2C AT 2 A RESOLUTION
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BL19 |
Synchrotron site | ESRF |
Beamline | BL19 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02-09 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 91.020, 91.020, 105.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.214 |
Rwork | 0.214 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 24.370 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.82) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.089 |
Total number of observations | 215022 * |
Number of reflections | 33176 |
<I/σ(I)> | 12.6 |
Completeness [%] | 95.5 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5 | 100 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | potassium phosphate | 50 (mM) | |
2 | 1 | reservoir | PEG8000 | 8-12 (%) | |
3 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
5 | 1 | drop | Tris-HCl | 10 (mM) | |
6 | 1 | drop | 50 (mM) | ||
7 | 1 | drop | 1 (mM) | ||
8 | 1 | drop | dithiothreitol | 2 (mM) | |
9 | 1 | drop | protein | 15 (mg/ml) |