1A6A

THE STRUCTURE OF AN INTERMEDIATE IN CLASS II MHC MATURATION: CLIP BOUND TO HLA-DR3

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1994-07
Detector	FUJI
Spacegroup name	P 32 1 2
Unit cell lengths	78.450, 78.450, 159.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.750
R-factor	0.246
Rwork	0.246
R-free	0.32500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dlh
RMSD bond length	0.015
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.000
High resolution limit [Å]	2.750	2.750
Rmerge	0.071	0.314
Number of reflections	13021 *
<I/σ(I)>	6.7	2.3
Completeness [%]	96.9	97.2
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.5		conditions similar to: Gorga, J.C., (1991) Res. Immun., 142, 401. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	12 (%)
2	1	1		100 (mM)
3	1	1	acetate	100 (mM)