1A5H

CATALYTIC DOMAIN OF HUMAN TWO-CHAIN TISSUE PLASMINOGEN ACTIVATOR COMPLEX OF A BIS-BENZAMIDINE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	280
Detector technology	AREA DETECTOR
Collection date	1995-09-28
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	151.830, 60.500, 62.610
Unit cell angles	90.00, 110.50, 90.00

Refinement procedure

Resolution	7.000 - 2.900
R-factor	0.178
Rwork	0.178
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtf
RMSD bond length	0.008
RMSD bond angle	26.158 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *	3.100
High resolution limit [Å]	2.900	2.800
Rmerge	0.088	0.250
Total number of observations	25355 *
Number of reflections	12586
Completeness [%]	94.4	86.2 *
Redundancy	2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5 *	23 *	pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.8 (mg/ml)
2	1	drop	acetate	10 (mM)
3	1	drop	inhibitor	0.8 (mM)
4	1	reservoir	acetate	0.1 (M)
5	1	reservoir	ammonium acetate	0.1 (M)
6	1	reservoir	PEG4000	25 (%)