1A5C
FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE FROM PLASMODIUM FALCIPARUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Collection date | 1996-10-26 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 119.200, 119.200, 132.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fba |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.100 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 20677 | |
| <I/σ(I)> | 8.2 | 3.4 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 | VAPOR DIFFUSION: 2.0 M AMMONIUM SULFATE/5% 2-PROPANOL, pH 7.6, vapor diffusion |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 2 | 1 | reservoir | 2-propanol | 5 (%) |
