1A4Z
ALDEHYDE DEHYDROGENASE FROM BOVINE MITOCHONDRIA COMPLEX WITH NAD (REDUCED) AND SAMARIUM (III)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 122.600, 198.400, 91.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.750 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.30200 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.100 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | PHASES |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.830 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.080 | 0.180 |
| Total number of observations | 220626 * | |
| Number of reflections | 50128 | |
| <I/σ(I)> | 9.6 | 3.5 |
| Completeness [%] | 85.0 | 59 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 14% PEG 8000, 100 MM MES, PH 6.5, 0.2 MM MGCL2, 1MM NAD; THEN SOAKED IN 1 MM SMCL3 OVERNIGHT. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 100 (mM) | |
| 2 | 1 | drop | NAD+ | 1 (mM) | |
| 3 | 1 | drop | 0.2 (mM) | ||
| 4 | 1 | drop | PEG8000 | 14 (%(w/v)) | |
| 5 | 1 | drop | enzyme | 8 (mg/ml) |
