1A4Y
RIBONUCLEASE INHIBITOR-ANGIOGENIN COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87, 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.553, 105.605, 93.516 |
| Unit cell angles | 90.00, 107.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BNH (PIG RIBONUCLEASE INHIBITOR) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.094 | 0.436 |
| Total number of observations | 158593 * | |
| Number of reflections | 72355 | |
| <I/σ(I)> | 4.5 | 1.6 |
| Completeness [%] | 86.9 | 65.4 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 16 * | THE COMPLEX WAS CRYSTALLIZED FROM 10% PEG4000, 20MM SODIUM CITRATE (PH 4.2), 0.1 AMMONIUM SULPHATE AND 25 MM DTT. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | inhibitor | 15.6 (mg/ml) | |
| 2 | 1 | drop | protein | 5.0 (mg/ml) | |
| 3 | 1 | drop | dithiothreitol | 0.2 (M) | |
| 4 | 1 | drop | glycerol | 0.0063ml | |
| 5 | 1 | reservoir | PEG4000 | 10 (%) | |
| 6 | 1 | reservoir | sodium citrate | 20 (mM) | pH4.2 |
| 7 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 8 | 1 | reservoir | dithiothreitol | 25 (mM) |
