1A4E

CATALASE A FROM SACCHAROMYCES CEREVISIAE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG
Synchrotron site	EMBL/DESY, HAMBURG
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-02
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	184.172, 184.172, 304.977
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.154
Rwork	0.154
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cat
RMSD bond length	0.010
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.074	0.330
Total number of observations	1276062 *
Number of reflections	123230
<I/σ(I)>	25.12	5.69
Completeness [%]	97.7	95 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	2.5 (M)
2	1	drop	Tris	0.2 (M)
3	1	drop	protein	28 (mg/ml)