1A41
TYPE 1-TOPOISOMERASE CATALYTIC FRAGMENT FROM VACCINIA VIRUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 1997-07 |
Detector | ADSC |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.700, 81.700, 84.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.30900 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.908 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.049 * | 0.224 * |
Total number of observations | 114536 * | |
Number of reflections | 13123 | |
Completeness [%] | 96.1 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 | 20 * | pH 5.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 23 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 100 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 59 (%) | |
4 | 1 | reservoir | dithiothreitol | 4 (mM) |