1A3A
CRYSTAL STRUCTURE OF IIA MANNITOL FROM ESCHERICHIA COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.570, 66.010, 73.860 |
| Unit cell angles | 90.00, 99.76, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.24200 |
| Structure solution method | MULTIPLE ANOMALOUS DISPERSION |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.830 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.262 |
| Total number of observations | 195844 * | |
| Number of reflections | 45271 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 95.8 | 84.2 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 5 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | Na2 EDTA | 1 (mM) | |
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | reservoir | PEG4000 | 36-38 (%(w/v)) | |
| 7 | 1 | reservoir | Taps-NaOH | 0.1 (mM) | |
| 8 | 1 | reservoir | Na2 EDTA | 5 (mM) | |
| 9 | 1 | reservoir | 1 (mM) |
