Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1A35

HUMAN TOPOISOMERASE I/DNA COMPLEX

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X4A
Synchrotron siteNSLS
BeamlineX4A
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1996-06
DetectorFUJI
Spacegroup nameP 1 21 1
Unit cell lengths71.800, 66.300, 71.800
Unit cell angles90.00, 98.40, 90.00
Refinement procedure
Resolution20.000 - 2.500
R-factor0.209

*

Rwork0.209
R-free0.30800
Structure solution methodRIGID BODY REFINEMENT
Starting model (for MR)RECONSTITUTED HUMAN TOPOISOMERASE I COVALENT COMPLEX WITH 22 BASE PAIR DUPLEX DNA
RMSD bond length0.011
RMSD bond angle22.300

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.600
High resolution limit [Å]2.5002.500
Rmerge0.0770.269
Total number of observations78779

*

Number of reflections18834
Completeness [%]83.987.7
Redundancy4.24.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.5

*

22

*

pH 7.70, VAPOR DIFFUSION, SITTING DROP, temperature 295.00K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111TRIS-HCL
1013TRIS-HCL
1113MGCL2
1213PEG 400
211EDTA
311DTT
411MGCL2
511PEG 400
612TRIS-HCL
712MGCL2
812DTT
912PEG 400
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirPEG4008 (%)
101dropTris-HCl2 (mM)pH7.5
111dropdithiothreitol2 (mM)
21reservoir33.3 (mM)
31reservoirTris35.5 (mM)pH7.7
41reservoirdithiothreitol4.4 (mM)
51dropwater0.003ml
61dropoligo0.01 (mM)
71drop0.7 (mM)
81dropprotein1 (mg/ml)
91dropEDTA0.2 (mM)

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon