1A27
HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 C-TERMINAL DELETION MUTANT COMPLEXED WITH ESTRADIOL AND NADP+
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 1996-02 |
| Detector | ESRF |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.380, 48.540, 60.770 |
| Unit cell angles | 90.00, 115.13, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| Rwork | 0.210 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fdt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 15988 | |
| Completeness [%] | 70.6 | 34.5 |
| Redundancy | 2.83 | 1.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | PROTEIN WAS CRYSTALLIZED FROM 12-16 % PEG4000, 100 MM HEPES PH 7.1, 0.5 MM ESTRADIOL, 200 MM NACL, 100MM MGCL2, 2.2 MM DECYL-BETA-D-MALTOSIDE; THEN SOAKED IN 30 % PEG 4000, 100 MM HEPES PH 7.1, 100 MM MGCL2, 0.5 MM ESTRADIOL, 1 MM NADP+ |
