1A0I
ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7 COMPLEX WITH ATP
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.700, 85.200, 79.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.600 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.34100 |
Structure solution method | MIR |
RMSD bond length | 0.012 |
RMSD bond angle | 3.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.055 | 0.114 |
Number of reflections | 13758 | |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.9 * | 20 * | Doherty, A.J., (1996) J. Biol. Chem., 271, 11083. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | drop | Bis Tris-HCl | 50 (mM) | |
3 | 1 | drop | EDTA | 2 (mM) | |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | Bis Tris-HCl | 100 (mM) | |
6 | 1 | reservoir | 100 (mM) | ||
7 | 1 | reservoir | methoxyPEG5000 | 12 (%(w/v)) |