1A02
STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 64.660, 85.460, 83.370 |
| 格子定数 [度] | 90.00, 112.03, 90.00 |
精密化法
| 残基 | 10.000 - 2.700 |
| R因子 | 0.246 |
| Rwork | 0.246 |
| R-free | 0.30300 |
| Structure solution method | MIR/MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 * |
| 結合角の平均二乗偏差(RMSD) [度] | 1.620 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.800 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| Rmerge_l_obs | 0.080 * | 0.430 * |
| 独立反射数 | 22079 | |
| 完全性 [%] | 98.3 | 93.3 |
| 冗長性 | 3.1 | 2.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | THE COMPLEX WAS CRYSTALLIZED IN 300-400 MM AMMONIUM ACETATE SALT, PH 7.5 (10 MM)., VAPOR DIFFUSION, HANGING DROP |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | HEPES | ||
| 10 | 1 | 2 | AMMINO ACETATE | ||
| 2 | 1 | 1 | DTT | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | GLYCEROL | ||
| 5 | 1 | 1 | AMMINO ACETATE | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | DTT | ||
| 8 | 1 | 2 | NACL | ||
| 9 | 1 | 2 | GLYCEROL |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | DNA | 0.2-0.4 (mM) | |
| 2 | 1 | reservoir | HEPES | 10 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | glycerol | 20 (%) | |
| 6 | 1 | reservoir | 300 (mM) |
