1ZVR
Crystal Structure of MTMR2 in complex with phosphatidylinositol 3,5-bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.180, 66.180, 262.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.980 |
| Rwork | 0.228 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1LW3 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 40385 | |
| <I/σ(I)> | 32.3 | 2.1 |
| Completeness [%] | 96.7 | 63.4 |
| Redundancy | 12 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 10,000, Tris, TCEP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
