1ZVF
The crystal structure of 3-hydroxyanthranilate 3,4-dioxygenase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.979, 0.9798, 0.9802, 0.9500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.104, 66.195, 130.188 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.410 |
R-factor | 0.2203 |
Rwork | 0.217 |
R-free | 0.26750 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.310 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.490 | 2.400 |
Rmerge | 0.082 | 0.065 | 0.244 |
Number of reflections | 13576 | ||
Completeness [%] | 93.2 | ||
Redundancy | 12.3 | 11.6 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8.5 | 293 | 30% PEG4000, 0.2M NaAc, 0.1M Tris_HCl, pH 8.5, hanging drop, temperature 293K |
1 | hanging drop | 8.5 | 293 | 30% PEG4000, 0.2M NaAc, 0.1M Tris_HCl, pH 8.5, hanging drop, temperature 293K |
1 | hanging drop | 8.5 | 293 | 30% PEG4000, 0.2M NaAc, 0.1M Tris_HCl, pH 8.5, hanging drop, temperature 293K |