1ZVD

Regulation of Smurf2 Ubiquitin Ligase Activity by Anchoring the E2 to the HECT domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	200
Collection date	2002-04-12
Wavelength(s)	0.9799, 0.9997, 0.9568
Spacegroup name	P 21 21 21
Unit cell lengths	51.359, 70.509, 152.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.570 - 2.100
Rwork	0.202
R-free	0.24792
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	1.883
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.600
High resolution limit [Å]	2.100
Number of reflections	32394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	Sodium Phosphate, Potassium Phosphate, and Sodium Acetate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K