1ZV8
A structure-based mechanism of SARS virus membrane fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.054, 55.565, 71.678 |
| Unit cell angles | 81.26, 87.08, 84.10 |
Refinement procedure
| Resolution | 70.710 - 1.940 |
| R-factor | 0.20699 |
| Rwork | 0.203 |
| R-free | 0.27427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.264 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.700 | 2.020 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Rmerge | 0.055 | 0.387 |
| Number of reflections | 32684 | |
| <I/σ(I)> | 11.1 | 3.1 |
| Completeness [%] | 94.5 | 94.8 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | PEG 8000, zinc acetate, sodium cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
