1ZT5

C-terminal domain of Insulin-like Growth Factor Binding Protein-1 isolated from human amniotic fluid complexed with Iron(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-14
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	38.210, 59.600, 31.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.340 - 1.818
R-factor	0.22333
Rwork	0.222
R-free	0.26425
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.421
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.340	1.880
High resolution limit [Å]	1.818	1.818
Number of reflections	6773
<I/σ(I)>	8.9	5.6
Completeness [%]	97.0	98.3
Redundancy	3.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	dioxane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K