1ZNB

METALLO-BETA-LACTAMASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-05-02
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	78.298, 78.298, 140.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.850
R-factor	0.167
Rwork	0.167
R-free	0.24100
Structure solution method	MULTIWAVELENGTH ANOMALOUS DIFFRACTION (MAD):
RMSD bond length	0.020
RMSD bond angle	24.400 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	1.970
High resolution limit [Å]	1.815	1.850
Rmerge	0.077 *	0.398 *
Total number of observations	292398 *
Number of reflections	37905	5995 *
<I/σ(I)>	6.7	1.7
Completeness [%]	99.2	95.8
Redundancy	7.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-8 (mg/ml)
2	1	drop	HEPES	55 (mM)
3	1	drop		0.01 (mM)
4	1	drop	PEG2000	26-28 (%)
5	1	reservoir	PEG2000	26-28 (%)
6	1	reservoir	HEPES	0.1 (M)