1ZL3
Coupling of active site motions and RNA binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-07 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.433, 40.753, 78.803 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.800 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k8w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.091 | 0.341 |
| Number of reflections | 10935 | |
| <I/σ(I)> | 23.6 | 3.6 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 6.34 | 4.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | ammonium sulfate [1.7M], PEG400 [2%], Hepes-KOH pH 7.5 [0.1M] and TCEP [1 mM], pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
