1ZKK
Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-10-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9686, 0.9792, 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.960, 45.775, 94.435 |
| Unit cell angles | 89.22, 87.07, 90.72 |
Refinement procedure
| Resolution | 19.630 - 1.450 |
| R-factor | 0.17073 |
| Rwork | 0.169 |
| R-free | 0.19902 |
| Structure solution method | MAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.278 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 122425 | |
| Completeness [%] | 32.0 | 34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | pentaerythritol ethoxylate, ammonium sulfate, Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
