1ZJM

Human DNA Polymerase beta complexed with DNA containing an A-A mismatched primer terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-11-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.100, 79.100, 54.900
Unit cell angles	90.00, 105.90, 90.00

Refinement procedure

Resolution	30.330 - 2.100
R-factor	0.228
Rwork	0.228
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bpx
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.081	0.436
Number of reflections	24584
Completeness [%]	99.2	92.3
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	PEG 3350, Sodium Acetate, Imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Sodium Acetate
2	1	1	Imidazole
3	1	1	PEG 3350
4	1	2	Sodium Acetate
5	1	2	PEG 3350