1ZCZ

Crystal structure of Phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase (TM1249) from THERMOTOGA MARITIMA at 1.88 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-03
Detector	ADSC QUANTUM 210
Spacegroup name	P 1
Unit cell lengths	62.084, 58.630, 72.749
Unit cell angles	99.26, 96.89, 106.13

Refinement procedure

Resolution	50.000 - 1.880
R-factor	0.15849
Rwork	0.156
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m9n 1g8mA
RMSD bond length	0.013
RMSD bond angle	1.395
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.910
High resolution limit [Å]	1.880	5.100	1.880
Number of reflections	74289
<I/σ(I)>	25.65		0.0205
Completeness [%]	95.6		70.6
Redundancy	2.9	4.4	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	5	293	20.0% PEG-6000, 0.1M Citrate pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K