1ZCD

Crystal structure of the Na+/H+ antiporter NhaA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9717
Spacegroup name	P 21 21 21
Unit cell lengths	108.868, 121.718, 123.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.450
R-factor	0.3016
Rwork	0.316
R-free	0.31600
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.573
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.570
High resolution limit [Å]	3.450	3.450
Number of reflections	19993
<I/σ(I)>	30.5
Completeness [%]	91.2	64.1
Redundancy	18.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	279	PEG 400, MgCl2, KCl, citrate, VAPOR DIFFUSION, HANGING DROP, temperature 279K
1	VAPOR DIFFUSION, HANGING DROP	4	279	PEG 400, MgCl2, KCl, citrate, VAPOR DIFFUSION, HANGING DROP, temperature 279K
1	VAPOR DIFFUSION, HANGING DROP	4	279	PEG 400, MgCl2, KCl, citrate, VAPOR DIFFUSION, HANGING DROP, temperature 279K