1ZCA
Crystal structure of G alpha 12 in complex with GDP, Mg2+ and AlF4-
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.501, 85.230, 82.885 |
| Unit cell angles | 90.00, 106.09, 90.00 |
Refinement procedure
| Resolution | 29.100 - 2.900 |
| R-factor | 0.23895 |
| Rwork | 0.236 |
| R-free | 0.29608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zcb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 15297 | |
| <I/σ(I)> | 6.9 | 1.67 |
| Completeness [%] | 88.7 | |
| Redundancy | 2.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | PEG 8000 (14%), Sodium Citrate (100 mM), NaCl (50 mM), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
