1ZB7
Crystal Structure of Botulinum Neurotoxin Type G Light Chain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00863 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 178.866, 178.866, 80.864 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.810 - 2.350 |
| R-factor | 0.17364 |
| Rwork | 0.174 |
| R-free | 0.22216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f82 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.411 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 30233 | |
| <I/σ(I)> | 24.4 | 4.8 |
| Completeness [%] | 93.9 | 95.3 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 6.00% PEG 6000, 0.65M Lithium Chloride, 0.10M Sodium Citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
