1ZAN
Crystal structure of anti-NGF AD11 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.801, 69.354, 64.104 |
| Unit cell angles | 90.00, 117.02, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.700 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cic |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 47951 | |
| <I/σ(I)> | 5.8 | 5.5 |
| Completeness [%] | 97.2 | 78.4 |
| Redundancy | 6.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20% Peg4000, 600mM NaCl, 100mM BTP, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
