1Z8L
Crystal structure of prostate-specific membrane antigen, a tumor marker and peptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2818 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.865, 157.765, 133.839 |
| Unit cell angles | 90.00, 93.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| Rwork | 0.252 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1de4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.470 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 80077 | |
| Completeness [%] | 99.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Peg3350 and sodium thiocyanate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Peg3350 and sodium thiocyanate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
