1Z7L
Crystal structure of fragment of mouse ubiquitin-activating enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 216.123, 216.123, 196.092 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.277 |
| Rwork | 0.277 |
| R-free | 0.30800 |
| Structure solution method | MAD combine with SIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.840 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.071 | 0.230 |
| Number of reflections | 43074 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 98.6 | 92.1 |
| Redundancy | 5.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | 2mM TCEP, 1.8M ammonium sulfate, 30% glycerol, 5mM Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |
