1Z69
Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.810, 83.410, 99.170 |
| Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.610 |
| R-factor | 0.1851 |
| Rwork | 0.185 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f07 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 | 0.214 |
| Number of reflections | 40607 | |
| <I/σ(I)> | 14.2 | 6.8 |
| Completeness [%] | 97.1 | 86.4 |
| Redundancy | 3.96 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | Tris/HCl, PEG 4000, Isopropanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
