1Z55
Effect of alcohols on protein hydration
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.216, 79.216, 37.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.2181 |
| Rwork | 0.218 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lym |
| RMSD bond angle | 0.004 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.100 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.021 | |
| Number of reflections | 8824 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 88.7 | |
| Redundancy | 14.1 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | sodium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
