1Z3H

The exportin Cse1 in its cargo-free, cytoplasmic state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93
Spacegroup name	P 21 21 2
Unit cell lengths	162.520, 113.040, 122.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.000 - 3.100
R-factor	0.287
Rwork	0.241
R-free	0.28800
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.543
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.480
High resolution limit [Å]	3.300	3.300
Number of reflections	41661
<I/σ(I)>		5.5
Completeness [%]	98.5	98.5
Redundancy	13.2	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 8000, MgCl2, glycerol, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 8000, MgCl2, glycerol, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K