1Z26

Structure of Pyrococcus furiosus Argonaute with bound tungstate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2139
Spacegroup name	P 1 21 1
Unit cell lengths	69.100, 103.501, 73.225
Unit cell angles	90.00, 102.46, 90.00

Refinement procedure

Resolution	37.820 - 2.500
Rwork	0.224
R-free	0.28200
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	58718
<I/σ(I)>	26.4	3.2
Completeness [%]	98.7	98.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8		alcohol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 100K